Background conductivity independent counter flow preconcentration method for capillary electrophoresis
نویسندگان
چکیده
منابع مشابه
A stability indicating capillary electrophoresis method for analysis of buserelin
A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
متن کاملContactless conductivity detector for microchip capillary electrophoresis.
A microfabricated electrophoresis chip with an integrated contactless conductivity detection system is described. The new contactless conductivity microchip detector is based on placing two planar sensing aluminum film electrodes on the outer side of a poly(methyl methacrylate) (PMMA) microchip (without contacting the solution) and measuring the impedance of the solution in the separation chann...
متن کاملA stability indicating capillary electrophoresis method for analysis of buserelin
A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
متن کاملPneumatic Microvalve for Electrokinetic Sample Preconcentration and Capillary Electrophoresis Injection
Here we show that a closed pneumatic microvalve on a PDMS chip can serve as a semipermeable membrane under an applied potential, enabling current to pass through while blocking the passage of charged analytes. Enrichment of both anionic and cationic species has been demonstrated, and concentration factors of ~70 have been achieved in just 8 s. Once analytes are concentrated, the valve is briefl...
متن کاملApplication of Online Preconcentration Affinity Capillary Electrophoresis Method to Glycans Labeled with 8-Aminonaphthalene-1,3,6-trisulfonic
Capillary electrophoresis (CE) is a highly successful separation technique for the analysis of biomolecules. Capillaries with an i.d. of 50 – 100 μm have a high electrical resistance and a large surface area-to-volume ratio. Therefore, very high electrical fields (≈650 V/cm) can be applied. This results in short analysis times and high resolution of a mixture of closely related components withi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2017
ISSN: 0173-0835
DOI: 10.1002/elps.201700071